ABSTRACT
Objective: To investigate the effects of dexamethasone on transforming growth factor [TGF]-beta1/Smads signaling pathway in benign biliary stricture [BBS] fibroblasts
Study Design: An experimental study
Place and Duration of Study: Guizhou Medical University, Guiyang, Guizhou, China, from January to August 2016
Methodology: Fibroblasts derived from rabbit BBS model were cultured and identified, then treated by different concentration of dexamethasone [0.02, 0.1 and 0.5 mg/ml]. Dexamethasone-treated cells and non-treated control groups were incubated respectively for 48 hours. Cell proliferation was assessed using cell counting kit-8. Relative mRNA expression of TGF-beta1, Smad4 and Smad7 were assessed by quantitative RT-PCR. Protein expression of TGF-beta1 and Smad4 were investigated by Western blotting
Results: Treatment with dexamethasone significantly inhibited the proliferation of BBS fibroblasts, significantly attenuated both the mRNA and protein expression of TGF-beta1 and Smad4, and significantly up-regulated the mRNA expression of Smad7 in BBS fibroblasts [all p<0.05, 0.1-0.5 mg/ml], and exhibited in a dose-dependent manner
Conclusion: TGF-beta1/Smads signaling pathway may play an important role in BBS progression; dexamethasone significantly altered the expression of TGF-beta1/Smads signaling pathway and significantly inhibited cell proliferation in rabbit BBS fibroblasts. Therefore, dexamethasone may be a therapeutic option for the prevention of BBS
ABSTRACT
Objective: To investigate the effects of tetramethylpyrazine [TMP] on transforming growth factor-beta1 [TGF- beta1], alpha-smooth muscle actin [alpha-SMA], and neuronal regeneration related protein [P311] in benign biliary stricture fibroblasts of rabbit. Study Design: An experimental study. Place and Duration of Study: Guizhou Medical University, Guiyang, Guizhou, China, from April to December 2015
Methodology: Fibroblasts isolated from rabbits following benign biliary stricture were cultured and treated with different concentrations of TMF [0.08, 0.4. and 2.0 mg/ml]. TMP-treated cells and non-treated control groups were incubated for 48-hours, and proliferation was assessed using the cell counting kit-8 assay. The mRNA expressions of TGF-beta1, alpha-SMA, and P311 were assessed by quantitative RT-PCR. Protein expressions of TGF-beta1 and alpha-SMA were investigated by Western blotting
Results: Treatment with TMP significantly reduced the proliferation of benign biliary stricture fibroblasts, and significantly attenuated both the mRNA and protein expressions of TGF-beta1, alpha-SMA, and P311 [p <0.05] in a dose-dependent manner
Conclusion: TMP significantly reduced the proliferation of benign biliary stricture fibroblasts, and significantly down- regulated the mRNA/protein expression of TGF-beta1, alpha-SMA, and P311. Therefore, TMP may be a therapeutic option for the prevention of benign biliary stricture
ABSTRACT
A 34-year-old man admitted to our department with complex blunt pancreaticoduodenal injury after a car accident. The wall of the first, second, and third portions of the duodenum was extensively lacerated, and the pancreas was longitudinally transected along the superior mesenteric vein-portal vein trunk. The pancreatic head and the uncinate process were devitalized and the distal common bile duct and the proximal main pancreatic duct were completely detached from the Vater ampulla. The length of the stump of distal common bile located at the cut surface of remnant pancreas was approximately 0.6 cm. A simplified Kausch-Whipple's procedure was performed after debridement of the devitalized pancreatic head and resection of the damaged duodenum in which the stump of distal common bile duct and the pancreatic remnant were embedded into the jejunal loop. Postoperative wound abscess appeared that eventually recovered by conservative treatment. During 16 months follow-up the patient has been stable and healthy. A simplified pancreaticoduodenectomy is a safe alternative for the Whipple procedure in managing complex pancreaticoduodenal injury in a hemodynamically stable patient.
Subject(s)
Adult , Humans , Male , Accidents, Traffic , Duodenum , Wounds and Injuries , Pancreas , Wounds and Injuries , Pancreaticoduodenectomy , Methods , Wounds, Nonpenetrating , General SurgeryABSTRACT
Objective To evaluate surgical indications and methods for regional pancreatoduodenectomy combined with blood vessel reconstruction. Method Eight patients underwent pancreatoduodenectomy combined with superior mesenteric vein and portal vein (SMV/PV) resection and reconstruction from May 2001 to December 2004,respectively. Results The overall mortality was 0 during perioperative period,no complications occurred. Histological specimen examinations demonstrated adenocarcinoma of pancreas head in all patients. The resected endothelium or margins of the blood vessel and pancreas were microscopically tumor free in all cases. Patients were followed-up from six months to four years.Two patients were died within one year. Four patients had survived for more than two years. Conclusion Regional pancreatoduodenectomy combined with blood vessel reconstruction could increase tumor resection rate in properly selected patients, and could be performed safely without increased morbidity and mortality.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between mRNA expression of inducible nitric oxide synthase (iNOS) and organ injury, and the effect of Salviae Miltiorrhizae on iNOS mRNA in severe acute pancreas (SAP) rats.</p><p><b>METHODS</b>Forty-five Wistar rats were randomly divided into 3 groups, the model group (MG), the Salviae Miltiorrhizae group (SMG), and the control group (CG), with 15 rats in each group. Rats in MG and SMG were established to SAP model by intraductal injection with 5% sodium taurocholate in a dose of 1.0 ml/kg, while rats in CG were merely performed sham-operation. Immediately after modeling, rats in SMG were injected with Salviae Miltiorrhizae injection (SMI) 2 ml/kg every 6 h for 4 times in total, but to rats in other two groups same volume of normal saline were administered. The level of serum amylase (AMY), nitric oxide (NO) and the volume of ascites of rats were determined 24h after modeling, and intensity of iNOS mRNA expression in pancreas, lung and kidney were detected in the same time using in situ hybridization and image analysis. The pathologic change was observed as well.</p><p><b>RESULTS</b>The volume of ascites and serum levels of AMY and NO in MG were significantly higher then those in SMG and CG (P < 0.05 and P <0.01). The expression of iNOS mRNA in pancreas, lung and kidney increased in MG and SMG, it was significantly higher in MG than that in SMG. And the pathological change of pancreas, lung and kidney in SMG was milder than that in MG.</p><p><b>CONCLUSIONS</b>Over-expression of iNOS mRNA could cause excessive synthesis of NO, and lead to injury of pancreas, lung and kidney in SAP. Salviae Miltiorrhizae in early stage of the disease can inhibit the over-expression of iNOS mRNA to ameliorate the injury of the pancreas, lung and kidney.</p>